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Please use this identifier to cite or link to this item: https://repository.monashhealth.org/monashhealthjspui/handle/1/49913
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dc.contributor.authorJin D.-
dc.contributor.authorLoh K.L.-
dc.contributor.authorShamekhi T.-
dc.contributor.authorTing Y.T.-
dc.contributor.authorLim Kam Sian T.C.C.-
dc.contributor.authorRoest J.-
dc.contributor.authorOoi J.D.-
dc.contributor.authorVivian J.P.-
dc.contributor.authorFaridi P.-
dc.date.accessioned2023-07-13T06:40:52Z-
dc.date.available2023-07-13T06:40:52Z-
dc.date.copyright2023-
dc.date.issued2023-07-03en
dc.identifier.citationMethods in Molecular Biology. 2691 (pp 351-369), 2023. Date of Publication: 2023.-
dc.identifier.urihttps://repository.monashhealth.org/monashhealthjspui/handle/1/49913-
dc.description.abstractEpitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.Copyright © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.-
dc.publisherHumana Press Inc.-
dc.relation.ispartofMethods in Molecular Biology-
dc.subject.meshgene editing-
dc.subject.meshgene expression-
dc.subject.meshimmunotherapy-
dc.subject.meshLentivirus-
dc.subject.meshmalignant neoplasm-
dc.subject.meshprotein expression-
dc.subject.meshleukocyte antigen-
dc.titleEngineering Cell Lines for Specific Human Leukocyte Antigen Presentation.-
dc.typeChapter-
dc.identifier.affiliationMonash University - School of Clinical Sciences at Monash Health-
dc.identifier.affiliationCentre for Inflammatory Diseases at Monash Health-
dc.identifier.doihttp://monash.idm.oclc.org/login?url=https://dx.doi.org/10.1007/978-1-0716-3331-1_25-
dc.publisher.placeUnited States-
dc.identifier.pubmedid37355557 [https://www.ncbi.nlm.nih.gov/pubmed/?term=37355557]-
dc.identifier.institution(Jin, Shamekhi, Lim Kam Sian, Faridi) Department of Medicine, School of Clinical Sciences, Monash University, Clayton, VIC, Australia-
dc.identifier.institution(Loh, Ting, Ooi) Centre for Inflammatory Diseases, Department of Medicine, Monash University, Monash Medical Centre, Clayton, VIC, Australia-
dc.identifier.institution(Lim Kam Sian, Faridi) Monash Proteomics & Metabolomics Facility, Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia-
dc.identifier.institution(Roest, Vivian) St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia-
dc.identifier.institution(Vivian) Department of Medicine, The University of Melbourne, Melbourne, VIC, Australia-
dc.identifier.affiliationmhJin, Shamekhi, Lim Kam Sian, Faridi) Department of Medicine, School of Clinical Sciences, Monash University, Clayton, VIC, Australia-
dc.identifier.affiliationmh(Loh, Ting, Ooi) Centre for Inflammatory Diseases, Department of Medicine, Monash University, Monash Medical Centre, Clayton, VIC, Australia-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypeChapter-
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