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https://repository.monashhealth.org/monashhealthjspui/handle/1/49913| Chapter/Book title: | Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation. | Authors: | Jin D.;Loh K.L.;Shamekhi T.;Ting Y.T.;Lim Kam Sian T.C.C.;Roest J.;Ooi J.D.;Vivian J.P.;Faridi P. | Monash Health Department(s): | Monash University - School of Clinical Sciences at Monash Health Centre for Inflammatory Diseases at Monash Health |
Institution: | (Jin, Shamekhi, Lim Kam Sian, Faridi) Department of Medicine, School of Clinical Sciences, Monash University, Clayton, VIC, Australia (Loh, Ting, Ooi) Centre for Inflammatory Diseases, Department of Medicine, Monash University, Monash Medical Centre, Clayton, VIC, Australia (Lim Kam Sian, Faridi) Monash Proteomics & Metabolomics Facility, Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia (Roest, Vivian) St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia (Vivian) Department of Medicine, The University of Melbourne, Melbourne, VIC, Australia |
Publication year: | 3-Jul-2023 | Copyright year: | 2023 | Publisher: | Humana Press Inc. | Place of publication: | United States | Publication information: | Methods in Molecular Biology. 2691 (pp 351-369), 2023. Date of Publication: 2023. | Book title: | Methods in Molecular Biology | Abstract: | Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.Copyright © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. | DOI: | http://monash.idm.oclc.org/login?url=https://dx.doi.org/10.1007/978-1-0716-3331-1_25 | PubMed URL: | 37355557 [https://www.ncbi.nlm.nih.gov/pubmed/?term=37355557] | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/49913 | Type: | Chapter | Subjects: | gene editing gene expression immunotherapy Lentivirus malignant neoplasm protein expression leukocyte antigen |
| Appears in Collections: | Books and Book Chapters |
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